DNA

Part:BBa_K5061007

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


Golden Gate adapter for Test Plasmid (TP) Golden Gate cloning platform

This Golden Gate adapter contains two BsaI recognition sites in opposite direction.

It is present in our composite part BBa_K5061034 and allows rapid engineering using Golden Gate Assembly of Test Plasmid (TP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6


[edit]
Categories
Parameters
None